Fig. 2.

Poly(A) profiles vary between cultured cell types and between transcripts. Panel A shows an MPAT assay for MT-CO3 transcripts in a variety of cell types including primary fibroblasts, Hep G2, 143B osteosarcoma, neuroblastoma (SHSY5Y) and HeLa. Negative controls for the first and second PCR steps are always performed. Panel B indicates the variation in poly(A) tail length between different human mt-transcripts at steady state in Hep G2 cells. Transcripts are aligned at the zero 3′ extension (0); nucleotide positions of 0, 10, 25 and 50 3′ extensions are indicated. The MPAT methodology was performed as described in Ref. [20].