TABLE 5.
ROBUSTNESS OF THE METHOD
| Conditions | Value | As | tR (min) | % (mean±sem)a | RSD (%) |
|---|---|---|---|---|---|
| Mobile phase (% methanol, ±2%) | 58 | 1.11 | 6.352 | 99.9±0.1 | 0.10 |
| 60 | 1.10 | 6.350 | 99.8±0.1 | 0.07 | |
| 62 | 1.10 | 6.348 | 99.8±0.2 | 0.11 | |
| Column temperature (±5°) | 21 | 1.12 | 6.350 | 99.5±0.5 | 0.07 |
| 24 | 1.10 | 6.350 | 99.7±0.3 | 0.05 | |
| 27 | 1.10 | 6.349 | 99.7±0.2 | 0.11 | |
| Flow rate (ml/min) | 0.8 | 1.11 | 6.351 | 99.8±0.5 | 0.09 |
| 1.0 | 1.10 | 6.350 | 100.0±0.2 | 0.11 | |
| 1.2 | 1.10 | 6.349 | 99.6±0.3 | 0.14 | |
| Lichrosorb C8 (250×4.6mm, 5 μm) | 1.11 | 6.360 | 99.5±0.4 | 0.16 |
a
Sem is the standard error of the mean, The recommended chromatographic conditions were Spherisorb C18 (250×4.6 mm, 5 mm) column with methanolwater 60:40 (v/v) mobile phase at a flow rate of 1 ml/min, UV detection at 254 nm and column temperature 24°