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. 2010 Dec 15;24(24):2748–2753. doi: 10.1101/gad.1995910

Figure 2.

Figure 2.

Genetic evidence that miR-14 acts via sugarbabe. (A,B,F) Ratio of total body triglyceride to total body protein. (C,E) mRNA levels measured in flies of the indicated genotypes. Data are presented as mean ± SD based on three independent biological replicates. P-values determined using Student's t-test. (A) Depletion of sugarbabe by expression of a UAS-RNAi transgene in IPCs reduced fat levels. P = 0.006 comparing dilp2-Gal4 UAS-GFP with dilp2-Gal4 UAS-sug-RNAi. (B) Overexpression of sugarbabe in IPCs increased fat levels. P = 0.002 comparing dilp2-Gal4/+ with dilp2-Gal4 UAS-sug. (C) Overexpression of sugarbabe reduced expression of ilp mRNAs. P = 1.2E-08 for ilp3 mRNA; P = 2.8E-05 for ilp5 mRNA. (D) sugarbabe expression in the adult IPCs using FISH (green). IPCs marked by dILP2-Gal4 nRFP (red). (E) sugarbabe mRNA levels from expression-profiling data. P = 0.0013 comparing control with miR-14 mutant; P = 0.0052 comparing miR-14 mutant with rescue-miR-14 mutant, dilp2-Gal4 UAS-miR-14. (F) Partial suppression of miR-14 mutant phenotype by IPC-specific depletion of sugarbabe. P = 1.96E-05 comparing control with miR-14 mutant, UAS-sug-RNAi (without the Gal4 driver, middle); P = 0.001 comparing miR-14 mutant, UAS-sug-RNAi with miR-14 mutant, dilp2-Gal4 UAS-sug-RNAi (with the Gal4 driver).