Figure 1.

Glutamine consumption is reduced in the absence of glucose. (A) IL-3-dependent bax^−/−bak^−/− cells and K562 cells were cultured in the presence or absence of glucose for 2 or 3 d and cell size was measured in femtoliters (fL) each day (mean ± SD of triplicates). Primary bone marrow cells were cultured in the presence or absence of glucose for 4 d and size was measured in femtoliters (mean ± SD of triplicates). (B) Transport of ¹⁴C-glutamine over 5 min was measured (mean ± SD of triplicates) after the following treatments: IL-3-dependent cells were cultured in the presence or absence of glucose or IL-3 for 2 d, K562 cells were cultured in the presence or absence of glucose for 2 d, and primary bone marrow cells were cultured in the presence or absence of glucose for 4 d. For all indicated panels, P < 0.05 (*), P < 0.005 (**), and P < 0.0005 (***).