Figure 2.

Dvl3 is requisite in Wnt5a/Fz2/cGMP/Ca²⁺signaling. A, Fz2 expressing cells were treated with control siRNA (-) or siRNA targeting individual Dvl isoforms. Cells were treated with vehicle (-Wnt5a), or Wnt5a (+Wnt5a) for 45 min and cellular cGMP was assayed (left panel). Suppression of Dvl1, 2 or 3 by using siRNA was examined by immunoblotting (right panel). B, cells were treated for 48 hr with either control siRNA (Ctr) or siRNA targeting individual Dvl isoforms. After loaded with Fura2, cells were challenged by Wnt5a (+Wnt5a). A representative graph of Ca²⁺ transient presented as A₃₄₀/A₃₈₀ for cells treated with different siRNA presented. Each line depicts the mean values obtained from 20 cells selected randomly.