Figure 5.

Sensitization of capsaicin responses by acute oxaliplatin treatment: A. Representative trace showing baseline 340/380 intracellular ratio in a DRG neuron and its increase in response to addition of 200 nM capsaicin (arrow) to test for capsaicin sensitivity. B. Following washout of medium, a second stimulus of 1 μM capsaicin (arrow) after 30 minutes gave a smaller second response compared with the first response (in A), due to desensitization. C. Same as in Figure 5A, showing traces 1,2,3,4 from 4 individual neurons, where trace 1 shows increased 340/380 ratio in response to added 200 nM capsaicin (arrow), but not traces 2,3 or 4. D. Incubation with 20 μg/ml oxaliplatin for 10 minutes after the first stimulus and washout significantly enhanced the response to 1 μM capsaicin (arrow, trace 1) and evoked responses in previously unresponsive neurons (traces 2 and 4).