Table 1.
Reverse primers1 used in 3 'RACE for amplification of 3' non-coding region of different ISAV segments using mRNA transcripts as template
| ISAV RNA segment | Primer sequence |
|---|---|
| Segment 1 | ssp3: 5'-CTGCTATGGAGAGGTGTATG-3' |
| Segment 2 | ssp3: 5'-GACTTGGACCCTCAACAGATC-3' |
| Segment 3 | ssp3: 5'-GCTAGAACATGTGTAGCTGTG-3' |
| Segment 4 | ssp3: 5'-TGGCAACATGACCCTGAGCT-3' |
| Segment 5 | ssp3: 5'-GAGTTCATCAAATGCTGTGGA-3' |
| Segment 6 | ssp3: 5'-GTTCCTCACTGATGCATTGAC-3' |
| Segment 7 | ssp3: 5'-GATGGTCTGTCACACATGC-3' |
| Segment 8 | ssp3: 5'-CTCTCTACTGTGTGATGAAAGA-3' |
1 The forward primer for PCR was the Universal Amplification Primer (UAP) that comes with the 3'RACE kit (Invitrogen).