. 2010 Nov 23;7:338. doi: 10.1186/1743-422X-7-338

Table 3.

Primers used in amplification of the non-coding region of different ISAV segments using cDNA from ligated mRNA transcripts as template

ISAV RNA segment	Primer sequence
Segment 1	Forward: 5'-CTGATGGATGAATATGGTGT-3'

	Reverse: 5'-GCTCCAACTGCTGCTCTAC-3'

Segment 2	Forward: 5'-CTTATCGACGAGGTGGAGGT-3'

	Reverse: 5'-CTCTGAAACCTGTTGCTACTAT-3'

Segment 3	Forward: 5'-GAGTTCGATGAGGACGATGA-3'

	Reverse: 5'-CTTCTGACAGGATTGCGCTG-3'

Segment 4	Forward: 5'-CAGAAAATTGCAGTCAGTACC-3'

	Reverse: 5'-CATGTGCTGATCGGTGTTGG-3'

Segment 5	Forward: 5'-GCTGATTGTAGTTGTGTTGGT-3'

	Reverse: 5'-GTCTGGTACAGAATGGAACG-3'

Segment 6	Forward: 5'-CATGTACAAGTCTAGAGGTAG-3'

	Reverse: 5'-GCGTTGTCCAGTGTCATCGA-3'

Segment 7	Forward: 5'-GCTTAGGGCTGGACTTCACT-3'

	Reverse: 5'-GCATGTGTGACAGACCATC-3'

Segment 8	Forward: 5'-GCATCCTGCTCGACAGAGAA-3'

	Reverse: 5'-CTTTCATCACACAGTAGAGAG-3'