Figure 3.
Quantitation of HIV-1 integration and 2-LTR circle formation in human macrophages. Macrophages were plated in 6-well plates with or without raltegravir treatment 24 h prior to infection, during infection and 48 h after infection (MOI = 0.1). (A) Seven days after infection, supernatant was assessed for p24 level of each group by p24 capture ELISA; (B) Six days after infection, cellular genomic DNA was extracted from macrophages, and HIV-1SX integration was measured by two-step quantitative PCR, and (C) 2-LTR circle formation was measured by real-time PCR. (**p < 0.01)