Table 2.

Inbreeding and temperature effects on knockout temperature.

Source of variation	d.f.	Mean Square	F	P
Covariate (water bath)	1	103.117	24.04	< 0.001
Karyotype (κ)	2	3.878	0.36	0.704
Cross ⊂ κ	15	11.027	2.57	0.001
Inbreeding (ι)	1	9.538	2.22	0.136
Temperature (τ)	1	77.034	17.96	< 0.001
Sex (ς)	1	154.979	36.13	< 0.001
κ × ι	2	4.176	0.97	0.378
κ × τ	2	1.999	0.47	0.628
κ × ς	2	8.106	1.89	0.152
ι × τ	1	1.047	0.24	0.621
ι × ς	1	0.435	0.10	0.750
τ × ς	1	0.022	0.01	0.943
κ × ι ×τ	2	7.798	1.82	0.163
κ × ι × ς	2	8.926	2.08	0.126
κ × τ ×ς	2	1.693	0.39	0.674
ι ×τ × ς	1	0.241	0.06	0.813
κ × ι × τ × ς	2	0.159	0.04	0.964
Error	668	4.289

Flies raised from inbred (isogenic) and outbred crosses of Drosophila subobscura reared at 18°C and 22°C. Karyotypes being compared are O_st/O_st, O₃₊₄/O₃₊₄, and O₃₊₄₊₈/O₃₊₄₊₈. (⊂ means "nested in".)