Figure 2.

Time-lapse imaging of the recruitment of Syt VII, Syt VII (C/S), CD63, and Lamp1 to nascent phagosomes. Syt VII^−/− BMMs transduced with adenovirus expressing CFP-CD63, Lamp1-RFP, and Syt VII–YFP or Syt VII (C/S)–YFP were exposed to 10 zymosan particles per cell and imaged by live confocal time-lapse microscopy. (A) Selected video frames showing that Syt VII–YFP and CFP-CD63 are simultaneously recruited to nascent phagosomes, followed by membrane tubules containing Lamp1-RFP (Video 1). (B) Selected video frames showing that CFP-CD63 is recruited to nascent phagosomes, but Syt VII (C/S)–YFP is not (Video 2). The differential interference contrast (DIC)–, YFP-, CFP-, and RFP-dissociated images for each highlighted phagosome (inset boxes) are shown on the right. Bars, 6 µm.