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. 2010 Nov 1;191(3):523–535. doi: 10.1083/jcb.201006022

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© 2010 Yu et al.

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Figure 3. — Orai1 internalization: ARF6, cholesterol, and Rab4. (A) Oocytes and eggs expressing 2 ng GFP-Orai1 alone or with 20 ng constitutively active ARF6 (Q67L) or 20 ng dominant-negative ARF6 (T22N). Orthogonal sections and deep-view images are shown. (A) GFP-Orai1–expressing oocytes were untreated, treated with 20 mM MβCD to deplete cholesterol, or treated with MβCD plus 5 mg/ml cholesterol for 9 h in Ringer, and then matured. (C) Orthogonal sections and deep-view images from eggs expressing 20 ng GFP-Orai1 and dominant-negative Rab4 mutants N121I or S22N. Bars, 5 µm.