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. 2010 Nov 1;191(3):523–535. doi: 10.1083/jcb.201006022

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© 2010 Yu et al.

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Figure 4. — Orai1-recycling endocytic pathway. (A) Oocytes expressing 10 ng GFP-Orai1 and RFP-Rab5 were untreated or injected with dynamin (Dyn) K44A and allowed 24 h to express. Deep-view images and orthogonal sections are shown. The number of Rab5- and Orai1-positive endosomes was measured using MetaMorph (n = 9–19). Error bars indicate mean ± SEM. (B) C3 exoenzyme was injected into GFP-Orai1– and RFP-Rab5–expressing oocytes 2 h before imaging. (C) Images GFP-Orai1–injected or GFP-Orai1– and Rab5 (S34N)–coinjected oocytes. Bars, 5 µm.