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. 2010 Nov 1;191(3):523–535. doi: 10.1083/jcb.201006022

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© 2010 Yu et al.

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Figure 5. — Cav is required for Orai1 internalization. (A) 10 ng GFP-Orai1– and mCherry-Cav–expressing oocytes and eggs. Images are ∼2-µm deep into the cell. Orthogonal sections are also shown. (B) GFP-Orai1 was allowed to express and traffic normally for 24 h followed by expression of 10 ng of the dominant-negative Cav mutant P168L 24 h before maturation. Bars, 5 µm.