Figure 5.

Depletion of Pom121 reduces cellular levels of Nup155. HeLa cells were incubated in the presence of control siRNAs (si-ctr) or siRNAs targeting Pom121, NDC1, or gp210 as indicated. (A) Cells were harvested and analyzed by Western blotting (WB) using antibodies directed against the indicated proteins. α-HuR was used as a loading control. (B) HeLa cells were processed for immunofluorescence microscopy using anti-Nup53, anti-Nup93, or anti-Nup107 polyclonal antibodies. Bar, 10 µm. (C) Cells were processed for immunofluorescence microscopy and interrogated using anti-Nup155 or mAb414 antibodies. Bar, 10 µm.