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. 2010 Nov 1;191(3):493–503. doi: 10.1083/jcb.201006092

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© 2010 Moore et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — ELM1 is not necessary for the SAC. Cells were arrested in G1 by treatment with mating pheromone (α factor) and then released into media containing 15 µg/ml nocodazole. Samples were collected at 45-min intervals, and the percentages of cells exhibiting multiple buds were scored. Each data point represents the mean percentage of multibudded cells from three separate experiments. At least 200 cells were scored per time point in each experiment. Strains: wild type (wt), yJC2295; elm1Δ, yJC5254; mad2Δ, yJC6497; bfa1Δ, yJC6447; bub2Δ, yJC5251; and kin4Δ, yJC6448.