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. 2010 Nov 1;191(3):645–659. doi: 10.1083/jcb.201004074

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© 2010 Maghzal et al.

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Figure 6. — EpCAM-induced tissue mixing is independent of β-cat/TCF signaling but involves down-regulation of PKC signaling. (A) Effect of dominant-negative xTCF (dnTCF) coexpression. dnTCF does not rescue EpCAM-induced cell mixing. (B) Effect of dnTCF on secondary axis induction by β-catenin. dnTCF completely abolished double-axis induction. **, P < 0.01, Student’s t test. (C) Rescue of EpCAM-induced mixing by PMA and Coleon U (ColU). EpCAM overexpressing BCRs or mesoderm explants were incubated in the presence of PMA/Coleon U for 15 min before the assay. * and **, P < 0.05 and P < 0.01, respectively, compared with EpCAM alone. (D) PKC inhibition interferes with tissue separation. Wild-type BCRs or mesoderm explants were preincubated for 15 min in the presence of 500 nM Bis1. The assay was then performed in the absence (second column) or in the presence (third column) of Bis1. * and **, P < 0.05 and P < 0.01, respectively, compared with controls. (E) Effect of PKC isoform-specific inhibitors on tissue separation. Inhibitors were added to the BCRs 15 min before assembling the assay. In the case of Gö6976, the assay was also then performed in the continuous presence of the inhibitor (last column). * and **, P < 0.05 and P < 0.01, respectively, compared with controls. Numbers on top indicate total number of explants/number of experiments.