FIGURE 5.

Activity-dependent degradation of ARMS/Kidins220 protein is not mediated by proteasomes and lysosomes. A, inhibition of proteasomes does not rescue activity-induced degradation of ARMS/Kidins220. Hippocampal cultures were depolarized with 50 mm KCl for 3 h in the presence of the proteasomal inhibitor epoxomicin or lactacystin, and ARMS/Kidins220 protein levels were assessed by Western blot. Actin is shown as a loading control. B, inhibition of lysosomes does not rescue activity-induced degradation of ARMS/Kidins220. Experiments were performed as in A using the lysosomal inhibitor chloroquine. Actin is shown as a loading control. C, quantification of ARMS/Kidins220 protein levels in A and B normalized to actin levels and plotted relative to untreated control (n ≥ three independent experiments; data are represented as the means ± S.E.).