FIGURE 2.

SelW promoter activity is up-regulated early in C2C12 myoblast differentiation. C2C12 myoblasts were transfected with −973SelW/Luc, MCK-Luc, or pGL3-basic and pRL-TK. Transfected cells were cultured in GM up to ∼50% confluence, followed by harvesting for SelW promoter analysis. For SelW promoter activity in DM, transfected C2C12 cells were grown up to ∼100% confluence and then transferred into DM to induce differentiation for 1 day prior to SelW luciferase analysis. Firefly luciferase activity was normalized to Renilla activity, and data are represented as means ± S.D. of three independent experiments performed in triplicate.