TABLE 3.

Substrate specificity of OOR

Substrate	Activity
	μmol min−1mg−1
Oxalate	0.049 ± 0.004
Glyoxylate	0.024 ± 0.006
Glyoxylate + 100 μm CoA	0.029 ± 0.007
Pyruvate	0.005 ± 0.001
Pyruvate + 100 μm CoA	0.005 ± 0.001
2-Oxobutyrate	0.014 ± 0.001
Oxaloacetate	0.007 ± 0.003
2-Oxovalerate	0.003 ± 0.001
2-Oxoglutarate	0.004 ± 0.002
Formate	NDa

^a ND, not detected. Slow enzyme-dependent methyl viologen reduction of about 0.002 unit/mg was seen in assays with no substrate, so we could not measure any slower activity.