FIGURE 1.
Bcl-2 or Bcl-xL overexpression inhibits heat-induced apoptosis. A, wild-type Jurkat cells (106/ml) were either left untreated or cultured for 1 h at 44 °C followed by a 6-h incubation at 37 °C and processed for cell death determination by flow cytometric analysis of annexin V-FITC and propidium iodide staining. B and C, duplicate aliquots of cells in A were harvested and processed for Western blotting (B) or caspase (DEVDase) activity determination (C). pro, pro-form; Casp, caspase; pro, pro-apoptotic; RFU, relative fluorescence units. D, control-transfected, Bcl-2-overexpressing, and Bcl-xL-overexpressing cells (106/ml) were left untreated or heated for 1 h at 44 °C followed by a 6-h incubation at 37 °C processed for cell death determination by flow cytometric analysis of annexin V-FITC and propidium iodide staining. In A and D, quadrants are defined as live (lower left), early apoptotic (lower right), late apoptotic (upper right), and necrotic (upper left). Numbers refer to the percentage of cells in each quadrant.