FIGURE 5.

PKC phosphorylates TRPC6 following a stimulation with CCh. A, metabolic labeling was carried out as described under “Experimental Procedures.” T6.11 cells were pretreated or not with 500 nm GF1 5 min prior to stimulation with 50 μm CCh. The autoradiogram is representative of three independent experiments. Autoradiography (upper panel) to assess phosphorylation or immunoblotting (lower panel) of TRPC6 was performed to determine the quantity of TRPC6 in the immunoprecipitate. B, densitometric analysis of the autoradiograms from C is with respect to the control condition. The histogram represents the average ± S.D. of three independent experiments. *, p < 0.05.