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. 2010 Oct 18;285(52):40554–40561. doi: 10.1074/jbc.M110.179481

FIGURE 5.

FIGURE 5.

Interaction of CAMDI with MRLC is required for radial migration. A, identification of SNP (R828W) in human CAMDI. B, reduced interaction of human CAMDI-R828W with MRLC and phosphomimetic MRLC. IP-IB assays indicated the reduced interaction of human CAMDI-R828W with MRLC-DD in SH-SY5Y cells (left). Note that the interaction of CAMDI-R828W with DISC1 is not changed. Amounts of protein expression in total lysates are shown below. C, CAMDI-R828W overexpression impairs radial migration. In utero electroporation experiments were performed with EGFP/control vector, EGFP/CAMDI-WT, or EGFP/CAMDI-R828W. The numbers of EGFP-positive neurons (CAMDI-WT, n = 950; CAMDI- R828W, n = 507) in the VZ, IZ, and CP are measured from three independent samples, and the average ratio (%) in each area is calculated in the right panel. Results represent the means ± S.E. Asterisks indicate significant difference between CAMDI-WT versus CAMDI R828W; ***, p < 0.001; **, p < 0.01; *, p < 0.05, Student's t test. Scale bar, 100 μm.