FIGURE 1.

Characterization of wild type rM180 and mutant amelogenins. A, CD spectra of amelogenins in 25 mm sodium acetate buffer, pH 5.8, at 25 °C. B, normalized fluorescence emission spectra of amelogenins at 25 °C. C, dependence of the hydrodynamic radii on the relative molecular mass for PMG-like subclass of IDPs taken from Ref. 36. An open circle and a star represent the hydrodynamic radii of rM180 and P41T mutant determined by DLS, respectively. D, ANS fluorescence in the presence of rM180 and P41T at 25 °C.