. 2010 Oct 7;285(52):40593–40603. doi: 10.1074/jbc.M110.131136

TABLE 2.

Thermodynamic parameters obtained from thermal denaturation of rM180 and mutant amelogenins

Data were analyzed based on Ref. 24.

Protein	ΔH_m^a	ΔS_m^b	T_m	ΔT_m^c	ΔΔG^d
	kcal/mol	cal/mol/K	°C	°C	kcal/mol
rM180	176 ± 7.1	562.9 ± 22.6	40.6 ± 0.1
T21I	102.9 ± 5.7	333.5 ± 18.5	35.3 ± 0.1	−5.3	−2.98
P41T	144.7 ± 5.8	466.1 ± 23.3	37.5 ± 0.2	−3.1	−1.75

^a ΔH_m indicates enthalpy of denaturation at T_m.

^b ΔS_m = ΔH_m/T_m.

^c ΔT_m = T_m_(mutant) − T_m_(rM180).

^d ΔΔG = 0.563 (ΔS_m for rM180 in kcal/mol) × ΔT_m. A negative value indicates a decrease in stability.