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Hoogsteen edge hydrogen bonding in the pol ι active site. A, template O6-methylG forms a bifurcated hydrogen bond with the protonated dCTP. B, template G forms two hydrogen bonds with the protonated dCTP (2ALZ). C, single hydrogen bond forms between template O6-methylG and incoming dTTP. D, bifurcated hydrogen bond does not form between O6-methylG and dTTP.
