FIGURE 3.

Reduction of Prx3 by Trx2 and Grx2 in vivo. A, monomer:dimer ratio of Prx3 detected by a specific 2-Cys peroxiredoxin redox blot in HeLa cells with siRNA-decreased expression levels of Grx2, Trx2, or both. Cells treated with unspecific siRNA (scr) were used as control. B, levels of oxidized Prx3 in transfected HeLa cells, constituted in percent. C, protein levels of Grx2 and Trx2 in HeLa cells treated with specific siRNA constructs, analyzed by a specific sandwich ELISA (Grx2) or Western blotting (Trx2), respectively. The average of ≥three independent experiments is shown.