FIGURE 2.

P. boydii conidia-induced IκBα degradation of MAPKs phosphorylation. Macrophages were either non-stimulated or stimulated with conidia (5 conidia/macrophage), LPS, or Pan₃Cys (both at 100 ng/ml) during the indicated periods of time. Cell extracts were prepared and submitted to electrophoresis. Detection of non-phosphorylated ERK1/2 was performed to normalize the amount of protein run on the lanes. A, IκBα degradation; B, ERK1/2; and C, p38 phosphorylation were detected by immunoblotting using anti-IκBα, anti-phospho-ERK1/2, or anti-phospho-p38 polyclonal antibodies, respectively. The figures are representative of two experiments with similar results.