FIGURE 2.

Respiratory enzyme complexes. A, Triton X-100-based BNGs demonstrate the fully assembled complex I in an isolated form (I) and in the supercomplex form (I:III). Complex I IGA is performed in a duplicate gel. B, native Western blot (native-Westerns) probed with anti-Rieske mAb (left) or with anti-ATPase subunit Va mAb (right, loading control in duplicate gel). S0, supercomplex I·III; S1, I·III·IV₁; S2, I·III·IV₂; S3, I·III·IV₃; S4, I·III·IV₄. C, quantitative analysis of the amount of fully assembled complexes I, III, and IV. Complexes I and IV were measured as densitometry scans of their IGAs on BNGs, complex III as densitometry scans of native Western blots. All values were normalized to complex V (see “Experimental Procedures”). Data are represented as mean ± S.E. from three to four independent experiments. An asterisk indicates statistical significance as p < 0.05 in comparison with wild type.