FIGURE 5.

The ability of two-dimensional BN/hrCNE to dissociate wild type and complex III mutant supercomplexes. A, Coomassie stain of the two-dimensional gel over the area of supercomplex dissociation. B, silver stain of the duplicates of the gels in A, which are cut from the 420-kDa region of the second dimension. This size is identical to that of IV₂. C, the amount of complex IV₂, measured by densitometry scanning of bands a–d in B, relative to the amount of Coomassie staining of monomeric complex V. Error bars represent mean ± S.E. from three independent experiments. An asterisk indicates statistical significance as p < 0.05 in comparison with wild type.