FIGURE 1.

The effect of protein phosphatase 2A/1 inhibitor on the levels of Ser-129-phosphorylated a-Syn in SH-SY5Y cells. SH-SY5Y cells stably expressing wild-type a-Syn (wt-aS/SH) were incubated in medium containing either 0.1% PBS, 0.1% DMSO or 10 nm OA for 4 h. A, cell lysates (20 μg/lane) were loaded on SDS-PAGE and analyzed by immunoblotting (IB) with anti-Ser-129-phosphorylated a-Syn antibody (psyn 64) and anti-total a-Syn antibody (Syn-1). For loading control, the same amounts of samples were immunoblotted with anti-β-actin antibody. Representative blots are shown. B, quantitative analysis of the alteration in the expression levels of Ser-129-phosphorylated a-Syn in cells in the absence or presence of OA. Relative ratios of the band intensity of Ser-129-phosphorylated a-Syn to β-actin and total a-Syn to β-actin are shown. Relative ratio was normalized to the starting material just before adding reagents. Data represent means ± S.D. and the p values (*, p < 0.05; **, p < 0.01) were estimated by unpaired Student's t test.