FIGURE 5.

RAGE interaction with short duplex DNA and RNA oligomers. A, native agarose gel electrophoresis. Oligomers of various concentrations were applied with or without 1 μm of RAGE12. No free oligomers were visible when RAGE12 was added to the wells. When applied to agarose gels, RAGE12 was consistently observed stay in or near the well, perhaps due to interaction with the agarose (data not shown). Therefore, RAGE12 bound oligomers were visible in the application wells when higher concentrations were used. B, gel filtration. Fifty microliters of 20 μm RAGE12 or 6.7 μm 19 bp dsDNA (same DNA as in A) or both together were injected onto a Superdex 200 10/30 column (GE Healthcare) equilibrated with 150 mm NaCl, 25 mm HEPES, pH 7.5. A repeatable elution shift was observed for both RAGE12 and dsDNA when injected together. Labels indicate the elution volumes (in ml) for each peak.