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. 2010 Oct 11;285(52):40852–40863. doi: 10.1074/jbc.M110.103812

FIGURE 2.

FIGURE 2.

GRHL2 transduction allows NHK to proliferate in the presence of elevated p16INK4A levels while attenuating the subculture-induced keratinocyte differentiation. A, NHOK (09-1) infected with LXSN or LXSN-GRHL2 were harvested at the indicated PD levels, and Western blotting was performed for GRHL2, PCNA, p53, p21WAF1, involucrin, and p16INK4A. β-Actin was probed as loading control. B, three independent cultures of NHOK (08-3, 08-4, and 919) were infected with LXSN or LXSN-GRHL2. Western blotting was performed with WCE obtained at the indicated PD levels for GRHL2, hTERT, PCNA, pRb, and p-Rb (Ser-807/811). β-Actin and CDK6 were used for loading controls. Densitometric analysis was performed and plotted.