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Duration of active telomerase is prolonged in NHOK by GRHL2 transduction. NHOK (09-1) infected with LXSN or LXSN-GRHL2 were harvested at the indicated PD levels and assayed for telomerase activity. WCE (1 μg) isolated in 1× CHAPS buffer was used for the PCR-based TRAP assay. The radioactively labeled PCR products were separated by 12.5% nondenaturing PAGE. ITAS, internal telomeric amplification standard.
