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. 2010 Oct 7;285(52):40864–40878. doi: 10.1074/jbc.M110.167296

FIGURE 1.

FIGURE 1.

Structures of the O- and N-glycans that are modified with 6-sulfo sialyl Lewis X structures, and a novel strategy for generating anti-carbohydrate mAbs. A, 6-sulfo sialyl Lewis X structures on O- and N-glycans. Core 2 branched O-glycan (upper left), extended core 1 structure (upper middle), biantennary O-glycan containing both a core 2 branch and extended core 1 structure (upper right), and N-glycans (bottom) can be modified with 6-sulfo sialyl Lewis X (shown in gray). The extended core 1 structure modified with GlcNAc-6-O-sulfate (shown in yellow) is recognized by the mAb MECA-79 (16). B, functional analysis of glycogenes. Glycogene-deficient (Gene A KO) mice and glycogene-overexpressing transfectant cells have been widely used for functional analyses. C, a novel strategy for generating anti-carbohydrate mAbs. Glycogene-deficient mice are immunized with glycogene-overexpressing transfected cells. The carbohydrate structures formed by the glycogene-encoded enzyme are expected to be highly antigenic in glycogene-deficient mice.