FIGURE 3.

Binding of S1 and S2 to sulfated and sialylated glycoproteins but not glycolipids. A, parental CHO (CHO-K1), CHO/CD34/F7/C1/C2/GlcNAc6ST-2, and CHO/CD34/F7/GlcNAc6ST-2 cells were stained with purified S1, S2, or MECA-79 using FITC-conjugated secondary Abs. B–D, the GlcNAc6ST-2 gene was transiently transfected into CHO-K1 (B), Lec1 (C), or Lec2 (D) cells with a pcDNA3.1/EGFP expression vector. Forty-eight hours after transfection, the binding of S1, S2, and MECA-79 was determined by flow cytometry, as described under “Experimental Procedures.” None, untransfected cells. Mock, cells mock-transfected with pcDNA3.1 empty vector together with the pcDNA3.1/EGFP expression vector. The data are representative of three (A and B) or two (C and D) independent experiments.