FIGURE 5.

Inhibition of the l-selectin-dependent adhesion of leukocytes to HEVs and leukocyte rolling by mAbs. A and B, binding of CMTMR-labeled leukocytes incubated with or without 10 μg/ml rat IgG or MEL-14 to PLN tissue sections incubated with or without 10 μg/ml mouse IgM, S1, S2, rat IgM, or MECA-79. A, photomicrographs of leukocyte binding to HEVs. Dotted lines, outline of HEV. Bar, 40 μm. B, number of bound leukocytes per HEV. The number of cells bound to an HEV is plotted. The number of HEVs analyzed per sample (n) is indicated at the bottom. The horizontal red lines represent the average number of leukocytes bound per HEV. N.S., not significant; *, p < 0.001. C, number of rolling leukocytes per 30 s on CHO transfectants stably expressing the 6-sulfo-sialyl Lewis X structure on N- and O-glycans (CHO/CD34/F7/C1/C2/GlcNAc6ST-2), pretreated with or without 10 μg/ml S1 or S2. Leukocytes applied to the flow chamber were treated with or without 10 μg/ml MEL-14. The data are representative of four (A and B) or three (C) independent experiments.