FIGURE 3.

Effects of overexpression or knockdown of CYP2B1 on complement-induced membrane permeabilization in cultured GECs. Negative (A and B) or CYP2B1 (C and D) adenovirus-infected GECs were incubated with anti-Fx1A followed by HIS (A and C) or FHS (B and D) and stained with calcein and ethidium homodimer as described under “Experimental Procedures.” In another set of experiments, CYP2B1-infected cells were treated with anti-Fx1A and FHS in the presence of scrambled (E) or CYP2B1-specific (F) siRNA. Fluorescence was detected as described under “Experimental Procedures.” Images are representative of three independent experiments. The extent of membrane permeabilization (red staining) is expressed as a percentage of total cell numbers.