FIGURE 2.

Direct binding of N-terminal WD40 repeats of tomosyn to synaptotagmin-1. A, schematic representation of tomosyn and its fragments used in this study. B, direct binding of the N-terminal WD40 repeats of tomosyn to synaptotagmin-1. MBP-tomosyn-N, MBP-tomosyn-C, or MBP alone was immobilized on amylose beads and incubated with ΔTM-synaptotagmin-1 in the absence of Ca²⁺. The bound proteins were eluted with SDS sample buffer and subjected to SDS-PAGE followed by CBB staining. The asterisk indicates degraded MBP-tomosyn-N. The result shown is representative of three independent experiments.