FIGURE 2.

Biosynthetic analysis of PCSK9 constructs. A, sequence representation of the prosegment, its Tyr³⁸ sulfation and Ser⁴⁷ phosphorylation, and the location of the two RRRREL mutants. The segment 31–60 was not seen in any of the crystal structures analyzed. B, various PCSK9-V5 constructs produced were co-expressed with the LDLR and analyzed by SDS-PAGE separation of mAb-V5 immunoprecipitated materials obtained following a 4-h pulse labeling with [³⁵S]Met + -Cys of HEK293 cells transiently overexpressing each construct.