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CIP75 co-localizes with wild-type and mutant Cx43. HeLa cells were transiently co-transfected with FLAG-CIP75 and ER-retained Cx43 (wild-type (A and E) or lysine mutants (B–D and F)). The subcellular localization of CIP75 (green) and Cx43 (red) with the ER marker calnexin (blue) was visualized by LSCM. CIP75 co-localizes with Cx43, regardless of the extent of lysine to arginine mutations. CIP75 and Cx43 co-localization occurs in an ER-like pattern. Scale bar, 10 μm.
