FIGURE 4.

Knockdown of GrnA blocks hepatic outgrowth but not specification. The expression levels of hhex (A–C) and prox1 (D–F) were examined using WISH in the controls and grnA morphants at 24 hpf (dorsal views, anterior up). The controls and grnA morphants were examined using WISH with hhex and prox1 (G–I and J–L) and fabp10 (M–O; maturation marker) at 72 hpf (dorsal views, anterior up). Co-injection of grnA mRNA (0.25 ng/embryo) and MO into one-cell embryos restored the deficient expression of the marker genes (I, L, and O). The arrowhead indicates the developing liver (A–C). Ctrl, control.