FIGURE 8.

GrnA positively modulates hepatic met expression in vitro and in vivo. Protein levels of GrnA, MET, ERK1/2, phosphorylated ERK1/2, β-catenin, and actin were examined by Western blot analysis in ZFL cells at 48 h post-grnA knockdown (A) and recombinant human PGRN treatment (B; 100 ng/ml). The relative GrnA, MET, ERK1/2, and β-catenin protein levels in each treatment were quantified as shown in the lower panel (C and D; normalized to the actin level). The expression of met in the liver region (E–G; arrowhead) was determined using WISH in controls, grnA morphants, and grnA-rescued embryos at 96 hpf. Ctrl, control. *, p < 0.05, t test. Error bars indicate S.D.