FIGURE 1.

BLT1 is required for FcγR-dependent phagocytosis in macrophages. A and B, Phagocytosis of opsonized zymosan particles (A) and nonopsonized particles (B) in BMMφ from wild-type (WT) and BLT1-deficient (KO) mice is shown. Number of bound or phagocytosed zymosan particles per macrophage was counted under a confocal laser scanning microscopy. Data represent the means ± S.E. *, p < 0.05; **, p < 0.001. C, engulfment of opsonized zymosan particles by BMMφ was observed using a confocal laser scanning microscope. Phagocytosed zymosan particles were observed as less bright spots (WT, −) than bright particles that represent zymosan particles bound on the cell surface. Differential interference contrast images are also presented to show similar numbers of the macrophages in each field. Representative photos are shown.