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LTB4-BLT1 signaling does not affect the expression of FcγRs. A–C, flow cytometric analysis of cell surface expression of FcγRs. BMMφ were incubated with or without PE-anti-mCD64 antibody (A, left panel), PE-anti-mCD16/CD32 antibody (A, right panel), mIgG2a-Alexa488 (B), opsonized zymosan-Alexa488 (C, left panel) and IgG-yellow-green beads (C, right panel). Where indicated, BMMφ were pretreated with LTB4 (100 nm, 30 min) prior to incubation with labeled antibodies (A and B).
