FIGURE 4.

LTB₄-dependent phagocytosis requires PI3K and Rac activation. A, effect of various inhibitors on phagocytosis in the presence or absence of LTB₄ in WT BMMφ. Cells were treated with inhibitors prior to the addition of LTB₄ and opsonized zymosan. Number of bound and phagocytosed zymosan was counted under a confocal laser scanning microscopy. Data represent the means ± S.E. *, p < 0.05; **, p < 0.001; ##, p < 0.001 versus #. B, effect of retroviral transfection of WT Rac or DN Rac on phagocytosis. Data represent the means ± S.E. *, p < 0.05; **, p < 0.001; ##, p < 0.001 versus #.