FIGURE 5.

LTB₄ signaling is sufficient to induce macrophage phagocytosis in the absence of FcγR signaling. A, effect of LTB₄ stimulation on phagocytosis in FcRγ-deficient BMMφ retrovirally transfected with rCD2-fused WT or mutant (YF) FcRγ. BMMφ expressing WT or mutant FcRγs were separated by MACS using a biotin anti-rCD2 antibody and subjected to phagocytosis assay. Data represent the means ± S.E. **, p < 0.001. B, effect of various inhibitors on LTB₄-dependent recovery of phagocytosis in FcRγ-deficient cells expressing FcRγ YF. Data represent the means ± S.E. **, p < 0.001.