FIGURE 6.

CaMKII associates with GSK-3. A, confocal images of CaMKIIβ co-localization with GSK-3α or GSK-3β. CGNs were labeled with antibodies against CaMKIIβ (green) and GSK-3α or GSK-3β (red) and then stained with Hoechst 33258 to visualize the nuclei (blue). Scale bar, 10 μm. B, CGN lysates were immunoprecipitated (IP) with CaMKIIβ antibody and processed for Western blot (WB) analysis using the indicated antibodies (left panel). CGN lysates were immunoprecipitated with GSK-3β antibody and processed for Western blot analysis to examine CaMKIIβ and GSK-3β (right panel). C, GFP-CaMKIIβ and V5-GSK-3α were co-transfected in HEK293A cells for 24 h. Cell extracts were immunoprecipitated with GFP antibody, and Western blot analyses were performed using anti-V5 and anti-GFP (left panel). Cell extracts were immunoprecipitated with V5 antibody, and Western blot analyses were performed using anti-GFP and anti-V5 (right panel). D, as described in C, and similar experiments were performed using V5-GSK-3β. All Input in this figure denotes 10% of total cell extracts.