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. 2010 Sep 13;285(52):41143–41151. doi: 10.1074/jbc.M110.153791

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — A, expression of HS^NS4F5 by HUVEC is increased after activation with TNF-α. Expression of HS^NS4F5 was measured by flow cytometry in nonactivated and TNF-α-activated HUVEC. Relative mean fluorescence intensity (MFI) is shown. The mean fluorescence intensity of nonactivated HUVEC is set at 100. The values are expressed as the means ± S.D. (n = 5). *, p < 0.05. B, HS^NS4F5 motifs on activated HUVEC contribute to firm leukocyte adhesion. Monolayers of HUVEC activated with TNF-α were coincubated with labeled 32Dcl3 (leukocyte) cells and antibody NS4F5. In addition, the effect of control antibody MPB49 was evaluated. The percentage of inhibition of adhesion is calculated by the formula [(100 − X)/100 × 100%], in which X is the relative adhesion compared with the control without addition (adhesion of 32Dcl3 cells without addition to activated HUVEC is set at 100%). The antibodies were used at 25 μg/ml. The values are expressed as the means ± S.D. (n = 5). *, p < 0.05 versus control without antibody.