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. 2010 Oct 26;285(52):41172–41186. doi: 10.1074/jbc.M110.167585

FIGURE 5.

FIGURE 5.

A, position of different SNPs in 1.8-kb promoter of −6A and −6G haplotypes of the hAGT gene. SNPs located at positions −6, −20, −217, −532, −793, −1074, −1178, −1561, −1562, and −1670 in −6A and −6G haplotypes of the hAGT gene promoter are shown. B, reporter construct containing 1.8 kb of hAGT gene promoter with −6A haplotype has 1.4-fold increased promoter activity as compared with −6G haplotype upon transfection in HepG2 cells. The empty box shows the promoter activity of −6A haplotype, and the filled box shows the promoter activity of −6G haplotype. C, reporter construct containing 1.8 kb of hAGT gene promoter with −6A haplotype has 4.7-fold increased glucocorticoid-induced promoter activity as compared with −6G haplotype upon transient transfection in HepG2 cells. Empty boxes show the promoter activity in the absence of Dex, and filled boxes show the promoter activity in the presence of Dex. D, reporter construct containing 1.8 kb of hAGT gene promoter with −6A haplotype has 1.5-fold increased promoter activity as compared with −6G haplotype upon transfection in HK-2 cells. The empty box shows the promoter activity of −6A haplotype, and the filled box shows the promoter activity of −6G haplotype. E, reporter construct containing 1.8 kb of the hAGT gene promoter with −6A haplotype has 3.8-fold increased glucocorticoid-induced promoter activity as compared with −6G haplotype upon transfection in HK-2 cells. Empty boxes show the promoter activity in the absence of Dex, and filled boxes show the promoter activity in the presence of Dex. Error bars, S.E.